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Anal Chem. 2012 Jan 17;84(2):956-62. doi: 10.1021/ac202047n. Epub 2011 Dec 28.

Development and certification of a standard reference material for vitamin D metabolites in human serum.

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Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States.


The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health's Office of Dietary Supplements (NIH-ODS), has developed a Standard Reference Material (SRM) for the determination of 25-hydroxyvitamin D [25(OH)D] in serum. SRM 972 Vitamin D in Human Serum consists of four serum pools with different levels of vitamin D metabolites and has certified and reference values for 25(OH)D(2), 25(OH)D(3), and 3-epi-25(OH)D(3). Value assignment of this SRM was accomplished using a combination of three isotope-dilution mass spectrometry approaches, with measurements performed at NIST and at the Centers for Disease Control and Prevention (CDC). Chromatographic resolution of the 3-epimer of 25(OH)D(3) proved to be essential for accurate determination of the metabolites.

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