Format

Send to

Choose Destination
See comment in PubMed Commons below
Anal Chem. 2012 Jan 17;84(2):956-62. doi: 10.1021/ac202047n. Epub 2011 Dec 28.

Development and certification of a standard reference material for vitamin D metabolites in human serum.

Author information

1
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States. karen.phinney@nist.gov

Abstract

The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health's Office of Dietary Supplements (NIH-ODS), has developed a Standard Reference Material (SRM) for the determination of 25-hydroxyvitamin D [25(OH)D] in serum. SRM 972 Vitamin D in Human Serum consists of four serum pools with different levels of vitamin D metabolites and has certified and reference values for 25(OH)D(2), 25(OH)D(3), and 3-epi-25(OH)D(3). Value assignment of this SRM was accomplished using a combination of three isotope-dilution mass spectrometry approaches, with measurements performed at NIST and at the Centers for Disease Control and Prevention (CDC). Chromatographic resolution of the 3-epimer of 25(OH)D(3) proved to be essential for accurate determination of the metabolites.

PMID:
22141317
PMCID:
PMC3287345
DOI:
10.1021/ac202047n
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society Icon for PubMed Central
    Loading ...
    Support Center