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Biochem Med (Zagreb). 2011;21(1):49-52.

Comparison of methods: Passing and Bablok regression.

Author information

1
Clinical Department of Laboratory Diagnostics, Clinical Hospital Centre and Department of Medical Informatics, Rijeka University School of Medicine, Rijeka, Croatia. lidija.bilic-zulle@medri.hr

Abstract

The comparison of methods experiment is important part in process of analytical methods and instruments validation. Passing and Bablok regression analysis is a statistical procedure that allows valuable estimation of analytical methods agreement and possible systematic bias between them. It is robust, non-parametric, non sensitive to distribution of errors and data outliers. Assumptions for proper application of Passing and Bablok regression are continuously distributed data and linear relationship between data measured by two analytical methods. Results are presented with scatter diagram and regression line, and regression equation where intercept represents constant and slope proportional measurement error. Confidence intervals of 95% of intercept and slope explain if their value differ from value zero (intercept) and value one (slope) only by chance, allowing conclusion of method agreement and correction action if necessary. Residual plot revealed outliers and identify possible non-linearity. Furthermore, cumulative sum linearity test is performed to investigate possible significant deviation from linearity between two sets of data. Non linear samples are not suitable for concluding on method agreement.

PMID:
22141206
[Indexed for MEDLINE]
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