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Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1501-5. doi: 10.1107/S1744309111042953. Epub 2011 Nov 29.

The structure of LsrB from Yersinia pestis complexed with autoinducer-2.

Author information

1
Microbiology Department, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.

Abstract

The crystal structure of LsrB from Yersinia pestis complexed with autoinducer-2 (AI-2; space group P2(1)2(1)2(1), unit-cell parameters a = 40.61, b = 61.03, c = 125.23 Å) has been solved by molecular replacement using the structure of LsrB from Salmonella typhimurium (PDB entry 1tjy) and refined to R = 0.180 (R(free) = 0.213) at 1.75 Å resolution. The electron density for bound AI-2 and the stereochemistry of the AI-2-binding site are consistent with bound AI-2 adopting the (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran conformation, just as has been observed in the crystal structures of the Salmonella typhimurium and Sinorhizobium meliloti LsrB-AI-2 complexes.

PMID:
22139152
PMCID:
PMC3232125
DOI:
10.1107/S1744309111042953
[Indexed for MEDLINE]
Free PMC Article

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