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Nat Methods. 2011 Dec 4;9(1):81-3. doi: 10.1038/nmeth.1802.

Controlled gene expression in primary Lgr5 organoid cultures.

Author information

1
Hubrecht Institute for Developmental Biology and Stem Cell Research, and University Medical Centre, Utrecht, The Netherlands.

Abstract

The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.

PMID:
22138822
DOI:
10.1038/nmeth.1802
[Indexed for MEDLINE]

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