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Methods Mol Biol. 2012;815:253-63. doi: 10.1007/978-1-61779-424-7_19.

Bioluminescence resonance energy transfer: an emerging tool for the detection of protein-protein interaction in living cells.

Author information

1
Department of Molecular Pediatrics, Dr. von Hauner Children's Hospital, Ludwig-Maximilians-University, M√ľnich 80337, Germany. soeren.gersting@med.lmu.de

Abstract

In the field of proteomics, numerous advanced technologies have evolved that aim to provide the molecular data necessary for an in-depth understanding of biological processes. Protein-protein interactions (PPI) are at the heart of cellular function and a milestone yet to be achieved is the mapping of a complete human interactome. Bioluminescence resonance energy transfer (BRET) has become a popular technique to investigate PPI. As BRET enables the detection of PPI in living cells, problems associated with in vitro biochemical assays can be circumvented, thus making BRET a powerful tool for monitoring interactions of virtually all kinds of protein species.

PMID:
22130997
DOI:
10.1007/978-1-61779-424-7_19
[Indexed for MEDLINE]

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