Format

Send to

Choose Destination
See comment in PubMed Commons below
Nucleus. 2011 Nov-Dec;2(6):591-600. doi: 10.4161/nucl.2.6.18044. Epub 2011 Nov 1.

Chromatin maintenance by a molecular motor protein.

Author information

1
Medical Sciences Program, School of Medicine, Indiana University, Bloomington, IN, USA. manjmazu@iupui.edu

Abstract

The kinesin motor protein KIF4 performs essential functions in mitosis. Like other mitotic kinesins, loss of KIF4 causes spindle defects, aneuploidy, genomic instability and ultimately tumor formation. However, KIF4 is unique among molecular motors in that it resides in the cell nucleus throughout interphase, suggesting a non-mitotic function as well. Here we identify a novel cellular function for a molecular motor protein by demonstrating that KIF4 acts as a modulator of large-scale chromatin architecture during interphase. KIF4 binds globally to chromatin and its absence leads to chromatin decondensation and loss of heterochromatin domains. KIF4-dependent chromatin decondensation has functional consequences by causing replication defects and global mis-regulation of gene expression programs. KIF4 exerts its function in chromatin architecture via regulation of ADP-ribosylation of core and linker histones and by physical interaction and recruitment of chromatin assembly proteins during S-phase. These observations document a novel function for a molecular motor protein in establishment and maintenance of higher order chromatin structure.

PMID:
22130187
PMCID:
PMC3324347
DOI:
10.4161/nucl.2.6.18044
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Taylor & Francis Icon for PubMed Central
    Loading ...
    Support Center