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Plant Cell. 2011 Nov;23(11):3871-8. doi: 10.1105/tpc.111.090779. Epub 2011 Nov 30.

Cautionary notes on the use of C-terminal BAK1 fusion proteins for functional studies.

Author information

1
The Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom.

Abstract

Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.

PMID:
22129600
PMCID:
PMC3246322
DOI:
10.1105/tpc.111.090779
[Indexed for MEDLINE]
Free PMC Article

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