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Nat Methods. 2011 Nov 27;9(1):78-80. doi: 10.1038/nmeth.1781.

Decoding cell lineage from acquired mutations using arbitrary deep sequencing.

Author information

1
Department of Pathology, University of Washington School of Medicine, Seattle, Washington, USA.

Abstract

Because mutations are inevitable, the genome of each cell in a multicellular organism becomes unique and therefore encodes a record of its ancestry. Here we coupled arbitrary single primer PCR with next-generation DNA sequencing to catalog mutations and deconvolve the phylogeny of cultured mouse cells. This study helps pave the way toward construction of retrospective cell-fate maps based on mutations accumulating in genomes of somatic cells.

PMID:
22120468
PMCID:
PMC3248619
DOI:
10.1038/nmeth.1781
[Indexed for MEDLINE]
Free PMC Article

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