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Curr Opin Chem Biol. 2011 Dec;15(6):813-21. doi: 10.1016/j.cbpa.2011.10.009. Epub 2011 Nov 24.

A starter kit for point-localization super-resolution imaging.

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1
Laboratory of Experimental Biophysics, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. suliana.manley@epfl.ch

Abstract

Super-resolution fluorescence imaging can be achieved through the localization of single molecules. By using suitable dyes, optical configurations, and software, it is possible to study a wide variety of biological systems. Here, we summarize the different approaches to labeling proteins. We review proven imaging modalities, and the features of freely available software. Finally, we give an overview of some biological applications. We conclude by synthesizing these different technical aspects into recommendations for standards that the field might apply to ensure quality of images and comparability of algorithms and dyes.

PMID:
22119536
DOI:
10.1016/j.cbpa.2011.10.009
[Indexed for MEDLINE]
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