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Methods Mol Biol. 2012;809:381-409. doi: 10.1007/978-1-61779-376-9_26.

Mapping protein-DNA and protein-protein interactions of ATP-dependent chromatin remodelers.

Author information

1
Southern Illinois University School of Medicine, Carbondale, IL, USA.

Abstract

Chromatin plays a key regulatory role in several DNA-dependent processes as it regulates DNA access to different protein factors. Several multisubunit protein complexes interact, modify, or mobilize nucleosomes: the basic unit of chromatin, from its original location in an ATP-dependent manner to facilitate processes, such as transcription, replication, repair, and recombination. Knowledge of the interactions of chromatin remodelers with nucleosomes is a crucial requirement to understand the mechanism of chromatin remodeling. Here, we describe several methods to analyze the interactions of multisubunit chromatin-remodeling enzymes with nucleosomes.

PMID:
22113290
DOI:
10.1007/978-1-61779-376-9_26
[Indexed for MEDLINE]

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