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PLoS One. 2011;6(11):e27737. doi: 10.1371/journal.pone.0027737. Epub 2011 Nov 16.

Ectopic expression reveals a conserved PHYB homolog in soybean.

Author information

1
Institute of Crop Sciences, National Key Facility of Crop Gene Resource and Genetic Improvement, Chinese Academy of Agricultural Sciences, Haidian District, Beijing, China.

Abstract

Phytochromes sense red/far-red light and trigger a cascade of physiological responses in plant. Here, a phytochrome B homolog, GmPHYB1, was amplified from the soybean genome, and its expression profiles were obtained for various parts of the plant and at various developmental stages. The gene was ectopically expressed in Arabidopsis thaliana, driven by CaMV 35S promoter, to study the physiological functions of the gene product. The overexpressors of GmPHYB1 behaved similarly to those of AtPHYB, but with some subtle differences with respect to the acceleration of flowering under short day conditions and the growth of the hypocotyl under certain light fluence rate. The results suggested that this soybean PHYB homolog was well conserved both at the level of sequence and physiological function.

PMID:
22110748
PMCID:
PMC3218029
DOI:
10.1371/journal.pone.0027737
[Indexed for MEDLINE]
Free PMC Article

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