Format

Send to

Choose Destination
See comment in PubMed Commons below
Exp Neurobiol. 2011 Sep;20(3):123-9. doi: 10.5607/en.2011.20.3.123. Epub 2011 Sep 20.

xCITED2 Induces Neural Genes in Animal Cap Explants of Xenopus Embryos.

Author information

1
Department of Biochemistry, College of Medicine, Hallym University, Chuncheon 200-702, Korea.

Abstract

Neural tissue is arisen from presumptive ectoderm via inhibition of bone morphogenetic protein (BMP) signaling during Xenopus early development. Previous studies demonstrate that ectopic expression of dominant negative BMP4 receptor (DNBR) produces neural tissue in animal cap explants (AC) and also increases the expression level of various genes involved in neurogenesis. To investigate detail mechanism of neurogenesis in transcriptional level, we analyzed RNAs increased by DNBR using total RNA sequencing analysis and identified several candidate genes. Among them, xCITED2 (Xenopus CBP/p300-interacting transcription activator) was induced 4.6 fold by DNBR and preferentially expressed in neural tissues at tadpole stage. Ectopic expression of xCITED2 induced anterior neural genes without mesoderm induction and reduced BMP downstream genes, an eye specific marker and posterior neural marker. Taken together, these results suggest that xCITED2 may have a role in the differentiation of anterior neural tissue during Xenopus early development.

KEYWORDS:

DNBR; Xenopus; neurogenesis; xCITED2

PubMed Commons home

PubMed Commons

0 comments

    Supplemental Content

    Full text links

    Icon for The Korean Society for Brain and Neural Science Icon for PubMed Central
    Loading ...
    Support Center