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Arch Microbiol. 2012 Jun;194(6):415-26. doi: 10.1007/s00203-011-0768-8. Epub 2011 Nov 23.

The biosynthesis of the polyether antibiotic nanchangmycin is controlled by two pathway-specific transcriptional activators.

Author information

1
State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China.

Abstract

The nanchangmycin (NAN) produced by Streptomyces nanchangensis NS3226 is a polyether antibiotic resembling monensin in their gene clusters and the chemical structures. They can inhibit gram-positive bacteria and be a growth promoter for ruminants. Within the nanchangmycin gene cluster (nan), we identified that two SARP-family regulatory genes, nanR1 and nanR2, were both required to activate the transcription of all nan polyketide genes. Overexpression of NanR1 and NanR2 in wild-type increase NAN yields by at least three folds. Bioinformatic analysis of the immediate upstream DNA sequence of each nan gene and quantitative real-time RT-PCR analysis of the nan operons identified five putative SARP binding sites. Moreover, deletion of an AraC-family repressor gene nanR4 increased expression of NanR1 and R2 and led to a threefold increase in NAN production.

PMID:
22109812
DOI:
10.1007/s00203-011-0768-8
[Indexed for MEDLINE]

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