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Br J Cancer. 2012 Jan 3;106(1):141-7. doi: 10.1038/bjc.2011.513. Epub 2011 Nov 22.

Clinicopathological significance of indoleamine 2,3-dioxygenase 1 expression in colorectal cancer.

Author information

1
Department of Pathology, Ghent University Hospital, Universiteit Gent, Block A, 5th floor, De Pintelaan 185, B-9000 Ghent, Belgium. Liesbeth.Ferdinande@UGent.be

Abstract

BACKGROUND:

Indoleamine 2,3-dioxygenase 1 (IDO1) is a tryptophan-catabolising enzyme that induces immune tolerance by modulating T-cell responses. Carcinomas may create an immunosuppressive state via IDO1 expression. Here we examined a possible contribution of IDO1 on this phenomenon and investigated whether IDO1 has prognostic value in colorectal cancer (CRC).

METHODS:

IDO1 expression was investigated by quantitative PCR and western blotting in three colon cancer cell lines, in basal state and after interferon (IFN)-γ stimulation. Semi-quantitative immunohistochemistry was used to evaluate IDO1 expression in 265 pT1-4N0-2Mx-staged CRCs. Results were related to clinical variables and correlated with amounts of CD3(+) and CD8(+) T lymphocytes, which were quantitatively evaluated using image analysis.

RESULTS:

In vitro expression of IDO1 depended on IFN-γ stimulation. Higher IDO1 expression at the tumour invasion front was an independent adverse prognostic factor in pT1-4N1Mx-staged CRC. It was associated with overall survival (P=0.001) and with metachronous metastases (P=0.018). IDO1 expression was not associated with the presence of CD3(+) or CD8(+) T lymphocytes.

CONCLUSION:

Higher IDO1 expression at the tumour invasion front is involved in CRC progression and correlates with impaired clinical outcome, suggesting that IDO1 is an independent prognostic indicator for CRC.

PMID:
22108515
PMCID:
PMC3251860
DOI:
10.1038/bjc.2011.513
[Indexed for MEDLINE]
Free PMC Article
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