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J Microbiol Methods. 2012 Jan;88(1):179-81. doi: 10.1016/j.mimet.2011.10.023. Epub 2011 Nov 12.

Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens.

Author information

1
Menzies School of Health Research, Charles Darwin University, Darwin, Australia.

Abstract

Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

PMID:
22108495
PMCID:
PMC3249147
DOI:
10.1016/j.mimet.2011.10.023
[Indexed for MEDLINE]
Free PMC Article

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