Sites of ubiquitin attachment in Saccharomyces cerevisiae

Proteomics. 2012 Jan;12(2):236-40. doi: 10.1002/pmic.201100166. Epub 2011 Dec 20.

Abstract

Sites of ubiquitin modification have been identified by mass spectrometry based on the increase in molecular mass of a tryptic peptide carrying two additional glycine residues from the ubiquitin moiety. However, such peptides with GG shifts have been difficult to discover. We identify 870 unique sites of ubiquitin attachment on 438 different proteins of the yeast Saccharomyces cerevisiae.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Affinity Labels / metabolism
  • Binding Sites
  • Cysteine / metabolism
  • Histidine / metabolism
  • Mass Spectrometry / methods
  • Peptides / isolation & purification
  • Peptides / metabolism*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Ubiquitin / metabolism*
  • Ubiquitin-Conjugating Enzymes / metabolism
  • Ubiquitination

Substances

  • Affinity Labels
  • Peptides
  • Saccharomyces cerevisiae Proteins
  • Ubiquitin
  • Histidine
  • Ubiquitin-Conjugating Enzymes
  • Cysteine