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J Biomech. 2012 Jan 3;45(1):123-8. doi: 10.1016/j.jbiomech.2011.09.007. Epub 2011 Nov 20.

Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers.

Author information

1
Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China.

Abstract

Human embryonic stem cells (hESC) and hESC-derived cardiomyocytes (hESC-CM) hold great promise for the treatment of cardiovascular diseases. However the mechanobiological properties of hESC and hESC-CM remains elusive. In this paper, we examined the dynamic and static micromechanical properties of hESC and hESC-CM, by manipulating via optical tweezers at the single-cell level. Theoretical approaches were developed to model the dynamic and static mechanical responses of cells during optical stretching. Our experiments showed that the mechanical stiffness of differentiated hESC-CM increased after cardiac differentiation. Such stiffening could associate with increasingly organized myofibrillar assembly that underlines the functional characteristics of hESC-CM. In summary, our findings lay the ground work for using hESC-CMs as models to study mechanical and contractile defects in heart diseases.

PMID:
22104169
DOI:
10.1016/j.jbiomech.2011.09.007
[Indexed for MEDLINE]

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