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Anal Chem. 2012 Jan 3;84(1):209-15. doi: 10.1021/ac202384v. Epub 2011 Dec 14.

Robust analysis of the yeast proteome under 50 kDa by molecular-mass-based fractionation and top-down mass spectrometry.

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1
Department of Chemistry, Proteomics Center of Excellence and Chemistry of Life Processes Institute, Northwestern University, 2145 North Sheridan Road, Evanston, Illinois 60208, USA.

Abstract

As the process of top-down mass spectrometry continues to mature, we benchmark the next installment of an improving methodology that incorporates a tube-gel electrophoresis (TGE) device to separate intact proteins by molecular mass. Top-down proteomics is accomplished in a robust fashion to yield the identification of hundreds of unique proteins, many of which correspond to multiple protein forms. The TGE platform separates 0-50 kDa proteins extracted from the yeast proteome into 12 fractions prior to automated nanocapillary LC-MS/MS in technical triplicate. The process may be completed in less than 72 h. From this study, 530 unique proteins and 1103 distinct protein species were identified and characterized, thus representing the highest coverage to date of the Saccharomyces cerevisiae proteome using top-down proteomics. The work signifies a significant step in the maturation of proteomics based on direct measurement and fragmentation of intact proteins.

PMID:
22103811
PMCID:
PMC3262231
DOI:
10.1021/ac202384v
[Indexed for MEDLINE]
Free PMC Article
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