Format

Send to

Choose Destination
See comment in PubMed Commons below
Nucleic Acids Res. 2012 Jan;40(Database issue):D1137-43. doi: 10.1093/nar/gkr973. Epub 2011 Nov 18.

The Human OligoGenome Resource: a database of oligonucleotide capture probes for resequencing target regions across the human genome.

Author information

1
Biomedical Informatics Training Program, Division of Oncology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, Stanford Genome Technology Center, Stanford University, Palo Alto and Department of Computer Science, Stanford University, Stanford, CA 94304, USA.

Abstract

Recent exponential growth in the throughput of next-generation DNA sequencing platforms has dramatically spurred the use of accessible and scalable targeted resequencing approaches. This includes candidate region diagnostic resequencing and novel variant validation from whole genome or exome sequencing analysis. We have previously demonstrated that selective genomic circularization is a robust in-solution approach for capturing and resequencing thousands of target human genome loci such as exons and regulatory sequences. To facilitate the design and production of customized capture assays for any given region in the human genome, we developed the Human OligoGenome Resource (http://oligogenome.stanford.edu/). This online database contains over 21 million capture oligonucleotide sequences. It enables one to create customized and highly multiplexed resequencing assays of target regions across the human genome and is not restricted to coding regions. In total, this resource provides 92.1% in silico coverage of the human genome. The online server allows researchers to download a complete repository of oligonucleotide probes and design customized capture assays to target multiple regions throughout the human genome. The website has query tools for selecting and evaluating capture oligonucleotides from specified genomic regions.

PMID:
22102592
PMCID:
PMC3245143
DOI:
10.1093/nar/gkr973
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Silverchair Information Systems Icon for PubMed Central
    Loading ...
    Support Center