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Nat Methods. 2011 Nov 20;9(1):72-4. doi: 10.1038/nmeth.1778.

Counting absolute numbers of molecules using unique molecular identifiers.

Author information

1
Genome-Scale Biology Program, Institute of Biomedicine, University of Helsinki, Helsinki, Finland.

Abstract

Counting individual RNA or DNA molecules is difficult because they are hard to copy quantitatively for detection. To overcome this limitation, we applied unique molecular identifiers (UMIs), which make each molecule in a population distinct, to genome-scale human karyotyping and mRNA sequencing in Drosophila melanogaster. Use of this method can improve accuracy of almost any next-generation sequencing method, including chromatin immunoprecipitation-sequencing, genome assembly, diagnostics and manufacturing-process control and monitoring.

PMID:
22101854
DOI:
10.1038/nmeth.1778
[Indexed for MEDLINE]
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