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Histopathology. 2011 Nov;59(5):993-1005. doi: 10.1111/j.1365-2559.2011.04015.x.

Improved detection of mycobacteria species in formalin-fixed tissue sections.

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1
TissuPath Specialist Pathology, Mount Waverley, Vic., Australia.

Abstract

AIMS:

To develop an antibody broadly reactive against mycobacterial species, which will improve detection of mycobacteria in tissue sections by immunohistochemistry (IHC).

METHODS:

A sheep antisera was developed by immunization with multiple mycobacteria, and was tested by IHC against a range of mycobacteria in tissues from many species, as well as negative tissue controls and other bacteria.

RESULTS:

The sheep antiserum, MAS-01, reacted with all 18 mycobacterial species tested, but did not react with uninfected inflammatory tissues. Although MAS-01 cross-reacted with two microbial genera which are related to mycobacteria (Corynebacteria and Proprionibacteria), it did not with Nocardia or Actinomyces. The antibody was more sensitive than the Ziehl-Neelsen stain for detection of tissue mycobacteria, and shortened the time required to identify these infections.

CONCLUSION:

The MAS-01 antiserum will facilitate rapid identification of tissue mycobacterial infection by histopathologists.

[Indexed for MEDLINE]

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