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Immunol Invest. 2012;41(3):261-74. doi: 10.3109/08820139.2011.626826. Epub 2011 Nov 16.

Six alkaloids inhibit secretion of IL-1α, TXB(2), ET-1 and E-selectin in LPS-induced endothelial cells.

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Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Jiangsu Nanjing 210014, PR China.


The aim of the research was to investigate the antiendotoxin effects of Sinomenine, Fangchinoline, Stachydrine, Chuanxionggzine, Oxymartrine and Evodiamine. Endothelial cells were challenged with 1 μg/mL LPS for 3 h then treated respectively with six alkaloids at three concentrations (1, 5 and 10 μg/mL). The cells were incubated at 37°C in a cell incubator for 21 h. The supernatants were collected and analyzed the levels of interleukin-1α (IL-1α), thromboxane B(2) (TXB(2)), endothelin-1 (ET-1) and E-selectin by ELISA kits. The results revealed that Sinomenine, Oxymartrine and Evodiamine inhibited the production of IL-1α; Stachydrine, Chuanxionggzine and Evodiamine inhibited the secretion of TXB(2); Sinomenine and Oxymartrine down-regulated ET-1 expression; Fangchinoline and Evodiamine decreased the level of E-selectin. All these changes were significant. Taken together, the data suggested that six alkaloids may effectively reduce inflammatory response via these cytokines.

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