(**A**) Scatter plot and bar graph of all 34 original subjects [14 R/LTS (green) and 20 NR/STS (purple)] used in the Discovery Study in connection with the F_{1} prognostic biomarker model. As can be seen, all 14 R/LTS subjects (green color) had F_{1} scores less than the determined cut-off score of 21.4, and, therefore, they were all identified correctly [for treatment response: sensitivity = 1.000; for survival: specificity = 1.000]. Regarding the NR/STS group (purple color), 19/20 had F_{1} scores greater than the determined cut-off score of 21.4, and, therefore, 19/20 were identified correctly [for treatment response: specificity = 19/20 = 0.950; for survival: sensitivity = 19/20 = 0.950]. For the Discovery Study, the ROC AUC of the F_{1} is 0.98929 with a 95% CI = [0.90449, 0.99884]. The mean F_{1} score of the 14 R/LTS subjects was 17.9358 (top of green bar) and the standard deviation (whisker above or below the top of the green bar) was 2.9622; whereas the mean F_{1} score of the 20 NR/STS subjects was 25.4697 (top of purple bar) and the standard deviation (whisker above or below the top of the purple bar) was 3.3651. The significance level was set at α = 0.001 (two-tailed), and the probability of significance was *P* = 1.30 × 10^{−7} (independent t-Test with T-value = −6.7405). The F_{1} is parametrically distributed with respect to both groups. (**B**) Scatter plot and bar graph of all 34 original subjects (14 R/LTS and 20 NR/STS) used in the Discovery Study in connection with the F_{2} and F_{3} prognostic biomarker models. As can be seen, 13/14 R/LTS subjects (green color) had F_{2} scores less than the determined cut-off score of 14.3, and, therefore, 13/14 subjects were identified correctly [for treatment response: sensitivity = 13/14 = 0.929; for survival: specificity = 13/14 = 0.929]. Regarding the NR/STS group (purple color), all 20 subjects had F_{2} scores greater than the determined cut-off score of 14.3, and, therefore, they were all identified correctly [for treatment response: specificity = 1.000; for survival: sensitivity = 1.000]. As can also be seen, all 14 R/LTS subjects (green color) had F_{3} scores less than the determined cut-off score of 14.7, and, therefore, they were all identified correctly [for treatment response: sensitivity = 1.000; for survival: specificity = 1.000]. Regarding the NR/STS group (purple color), 19/20 subjects had F_{3} scores greater than the determined cut-off score of 14.7, and, therefore, 19/20 subjects were identified correctly [for treatment response: specificity = 19/20 = 0.950; for survival: sensitivity = 19/20 = 0.950]. For the Discovery Study, the ROC AUC of the F_{2} is 0.98929 with a 95% CI = [0.90321, 0.99886], whereas the ROC AUC of the F_{3} is 0.98214 with a 95% CI = [0.86165, 0.99782]. The mean F_{2} score of the 14 R/LTS subjects was 13.4223 (top of green bar) and the standard deviation (whisker above or below the top of the green bar) was 0.8905; whereas the mean F_{2} score of the 20 NR/STS subjects was 15.1843 (top of purple bar) and the standard deviation (whisker above or below the top of the purple bar) was 0.6407. The mean F_{3} score of the 14 R/LTS subjects was 13.8864 and the standard deviation was 0.7017; whereas the mean F_{3} score of the 20 NR/STS subjects was 15.3433 and the standard deviation was 0.6082. The significance level was set at α = 0.001 (two-tailed), and the probability of significance for the F_{2} was *P* = 1.37 × 10^{−7} (independent t-Test with T-value = −6.7217), whereas the probability of significance for the F_{3} was *P* = 2.93 × 10^{−7} (independent t-Test with T-value = −6.4541). Both the F_{2} and the F_{3} are parametrically distributed with respect to both groups.

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