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Methods Mol Biol. 2012;813:85-106. doi: 10.1007/978-1-61779-412-4_5.

Reprogramming a GFP reporter gene subjects it to complex lentiviral gene regulation.

Author information

1
Life Technologies Corp/GeneArt AG, Regensburg, Germany.

Abstract

Late human immunodeficiency virus (HIV)-derived RNAs encoding relevant therapeutic targets or promising vaccine compounds, such as the HIV-1 group-specific antigen (Gag), are translocated from the nucleus into the cytoplasm via sophisticated export machinery. Relevant steps include the concerted action of several cis-acting RNA elements with the viral Rev-shuttle protein and several cellular components (Ran1/Exportin; Crm1). Based on detailed understanding of the molecular mechanisms guiding this complex process, we used rational codon usage modification to design and reprogram a GFP encoding reporter RNA now exactly mimicking the complex transcriptional and posttranscriptional regulation of late lentiviral mRNAs.

PMID:
22083737
DOI:
10.1007/978-1-61779-412-4_5
[Indexed for MEDLINE]

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