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Cell. 1990 Oct 5;63(1):97-107.

Reduced DNA polytenization of a minichromosome region undergoing position-effect variegation in Drosophila.

Author information

1
Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210.

Abstract

Molecular analysis of a Drosophila minichromosome, Dp(1;f)1187, revealed a relationship between position-effect variegation and the copy number reductions of heterochromatic sequences that occur in polytene cells. Heterochromatin adjacent to a defined junction with euchromatin underpolytenized at least 60-fold. Lesser reductions were observed in euchromatic sequences up to 103 kb from the breakpoint. The copy number changes behaved in all respects like the expression of yellow, a gene located within the affected region. Both copy number and yellow expression displayed a cell-by-cell mosaic pattern of reduction, and adding a Y chromosome, a known suppressor of variegation, increased both substantially. We discuss the possibility that changes in replication alter copy number locally and also propose an alternative model of position-effect variegation based on the somatic elimination of heterochromatic sequences.

PMID:
2208283
PMCID:
PMC3229194
DOI:
10.1016/0092-8674(90)90291-l
[Indexed for MEDLINE]
Free PMC Article

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