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Nat Methods. 2011 Nov 13;8(12):1056-8. doi: 10.1038/nmeth.1775.

Embryonic stem cell-based mapping of developmental transcriptional programs.

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1
Departments of Pathology, Neurology and Neuroscience, Center for Motor Neuron Biology and Disease and Columbia Stem Cell Initiative, Columbia University Medical Center, New York, New York, USA.

Abstract

The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.

PMID:
22081127
PMCID:
PMC3228994
DOI:
10.1038/nmeth.1775
[Indexed for MEDLINE]
Free PMC Article
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