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Science. 2011 Nov 11;334(6057):821-4. doi: 10.1126/science.1211915.

A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A.

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1
Department of Molecular Biology and Biotechnology, Krebs Institute, University of Sheffield, Sheffield S10 2TN, UK.

Abstract

The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.

PMID:
22076380
PMCID:
PMC3364511
DOI:
10.1126/science.1211915
[Indexed for MEDLINE]
Free PMC Article

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