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Dev Dyn. 2011 Dec;240(12):2646-56. doi: 10.1002/dvdy.22778.

Deficiency in Crumbs homolog 2 (Crb2) affects gastrulation and results in embryonic lethality in mice.

Author information

1
Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institutet, Stockholm, Sweden.

Erratum in

  • Dev Dyn. 2012 Feb;241(2):431. Pikkarainan, Timo [corrected to Pikkarainen, Timo].

Abstract

The Crumbs family of transmembrane proteins has an important role in the differentiation of the apical membrane domain in various cell types, regulating such processes as epithelial cell polarization. The mammalian Crumbs protein family is composed of three members. Here, we inactivated the mouse Crb2 gene with gene-targeting techniques and found that the protein is crucial for early embryonic development with severe abnormalities appearing in Crb2-deficient embryos at late-gastrulation. Our findings indicate that the primary defect in the mutant embryos is disturbed polarity of the epiblast cells at the primitive streak, which affects epithelial to mesenchymal transition (EMT) during gastrulation, resulting in impaired mesoderm and endoderm formation, and embryonic lethality by embryonic day 12.5. These findings therefore indicate a novel role for the Crumbs family of proteins.

PMID:
22072575
DOI:
10.1002/dvdy.22778
[Indexed for MEDLINE]
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