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Toxins (Basel). 2010 Feb;2(2):297-309. doi: 10.3390/toxins2020297. Epub 2010 Feb 25.

Detection of fumonisin b1 and ochratoxin a in grain products using microsphere-based fluid array immunoassays.

Author information

1
Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington DC, 20375, USA. george.anderson@nrl.navy.mil

Abstract

Grain products are a staple of diets worldwide and therefore, the ability to accurately and efficiently detect foodborne contaminants such as mycotoxins is of importance to everyone. Here we describe an indirect competitive fluid array fluoroimmunoassay to quantify the mycotoxins, fumonisin B1 and ochratoxin A. Both toxins were immobilized to the surface of microspheres using a variety of intermediate molecules and binding of biotinylated "tracer" antibody tracers determined through flow cytometry using streptavidin-phycoerythrin conjugates and the Luminex100 flow cytometer. Competitive assays were developed where the binding of biotinylated monoclonal antibodies to fumonisin B and ochratoxin A was competitively inhibited by different concentrations of those toxins in solution. Concentrations of fumonisin giving 50% inhibition were 300 pg/mL in buffer, 100 ng/g in spiked oats, and 1 μg/g in spiked cornmeal; analogous concentrations for ochratoxin A were 30 ng/mL in buffer, 30 ng/g in spiked oats, and 10 ng/g in spiked corn. The future challenge will be to expand the number of mycotoxins tested both individually and in multiplexed format using this platform.

KEYWORDS:

bead array; detection; fumonisin; immunoassay; mycotoxin; ochratoxin

PMID:
22069585
PMCID:
PMC3202808
DOI:
10.3390/toxins2020297
[Indexed for MEDLINE]
Free PMC Article
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