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Inflamm Res. 2012 Jan;61(1):79-85. doi: 10.1007/s00011-011-0394-6. Epub 2011 Nov 9.

Mast cells cultured from IL-3-treated mice show impaired responses to bacterial antigen stimulation.

Author information

1
Parasite Immune Modulation Group, School of Nursing and Human Sciences, Dublin City University, Glasnevin, Dublin, Ireland.

Abstract

OBJECTIVE AND DESIGN:

This study exploits the biological activity of interleukin (IL)-3 to generate high yields of peritoneal mast cells ex vivo in order to examine pro-inflammatory immune responses in ex-vivo culture.

MATERIAL OR SUBJECTS:

Mast cells were obtained from the peritoneal cavity of C57BL/6 mice.

TREATMENT:

Mice were injected intraperitoneally twice per day for 5 days with IL-3 (40-50 μg/ml) to increase mast cell numbers.

METHODS:

Histological studies examined mast cell numbers in the peritoneal cavity, intestine, lung, spleen and skeletal muscle. Peritoneal mast cells cultured ex vivo (PCMCs) were stimulated for 24 h with lipopolysaccharide and Bordetella pertussis antigen and secretion of tumour necrosis factor-α, IL-6, IL-4, IL-5, IL-10 and interferon-γ into supernatant was measured by commercial ELISA. Cell surface marker expression of FcεRI, c-kit, OX40L and TLR2 was measured by flow cytometry. Mast cell degranulation was measured using a β-hexosaminidase assay.

RESULTS:

IL-3 treatment increases mast cell numbers in the peritoneal cavity, spleen and muscle but not intestine and lung of C57BL/6 mice. PCMCs generated from IL-3-treated mice exhibit impaired growth, differentiation and responses to activation as measured by decreased cytokine secretion and cell surface marker expression.

CONCLUSION:

Mast cells cultured from IL-3-treated mice show impaired responses.

PMID:
22068549
DOI:
10.1007/s00011-011-0394-6
[Indexed for MEDLINE]

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