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Nucleic Acids Res. 2012 Mar;40(5):1904-15. doi: 10.1093/nar/gkr857. Epub 2011 Nov 8.

LSD1 cooperates with CTIP2 to promote HIV-1 transcriptional silencing.

Author information

1
University of Strasbourg, EA4438, Institute of parasitology, Strasbourg, France.

Erratum in

  • Nucleic Acids Res. . 2014 Apr;42(6):4141.

Abstract

Microglial cells are the main HIV-1 targets in the central nervous system (CNS) and constitute an important reservoir of latently infected cells. Establishment and persistence of these reservoirs rely on the chromatin structure of the integrated proviruses. We have previously demonstrated that the cellular cofactor CTIP2 forces heterochromatin formation and HIV-1 gene silencing by recruiting HDAC and HMT activities at the integrated viral promoter. In the present work, we report that the histone demethylase LSD1 represses HIV-1 transcription and viral expression in a synergistic manner with CTIP2. We show that recruitment of LSD1 at the HIV-1 proximal promoter is associated with both H3K4me3 and H3K9me3 epigenetic marks. Finally, our data suggest that LSD1-induced H3K4 trimethylation is linked to hSET1 recruitment at the integrated provirus.

PMID:
22067449
PMCID:
PMC3300010
DOI:
10.1093/nar/gkr857
[Indexed for MEDLINE]
Free PMC Article

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