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Methods Mol Biol. 2012;803:205-18. doi: 10.1007/978-1-61779-364-6_14.

Chemical cross-linking and high-resolution mass spectrometry to study protein-drug interactions.

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1
Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther-Universität Halle-Wittenberg, Halle (Saale), Germany.

Abstract

This method describes the combination of chemical cross-linking and high-resolution mass spectrometry for analyzing conformational changes in target proteins that are induced by drug binding. Our approach is exemplified for detecting conformational changes within the peroxisome proliferator-activated receptor alpha upon binding of low-molecular weight compounds, proving that our strategy provides a basis to efficiently characterize target protein-drug interactions.

PMID:
22065227
DOI:
10.1007/978-1-61779-364-6_14
[Indexed for MEDLINE]
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