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J Ethnopharmacol. 2012 Jan 6;139(1):110-8. doi: 10.1016/j.jep.2011.10.027. Epub 2011 Oct 29.

Identification of hypoglycaemic compounds from berries of Juniperus oxycedrus subsp. oxycedrus through bioactivity guided isolation technique.

Author information

1
Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, 06330, Etiler, Ankara, Turkey. nsendogdu@gazi.edu.tr

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Decoction of Juniperus oxycedrus subsp. oxycedrus L. (Cupressaceae) berries is used internally as tea and pounded fruits are consumed to lower blood glucose levels in Turkey.

AIM OF THE STUDY:

To evaluate hypoglycaemic and antidiabetic activity of J. oxycedrus subsp. oxycedrus berries and to identify active compounds through bioactivity guided isolation technique.

MATERIAL AND METHODS:

Hypoglycaemic effect of J. oxycedrus subsp. oxycedrus (Joso) berry extracts on oral administration was studied using in vivo models in normal, glucose-hyperglycaemic rats. Streptozotocin induced diabetic rats were used to examine antidiabetic activity of Joso extracts, subextracts, fractions, subfractions and shikimic acid (SA).

RESULTS:

Through in vivo bioactivity-guided fractionation processes, shikimic acid, 4-O-β-d-glucopyranosyl ferulic acid and oleuropeic acid-8-O-β-d-glucopyranoside were isolated from the n-butanol subextract by silica gel and reverse phase column chromatography as the main active ingredient of the active subfraction. After 8 days administration of the major compound shikimic acid, blood glucose levels (24%), malondialdehyde levels in kidney tissues (63-64%) and liver enzymes (AST, ALT, ALP) of diabetic rats were decreased.

CONCLUSION:

Results indicated that Joso berry extract and its active constituents might be beneficial for diabetes and its complications.

PMID:
22063725
DOI:
10.1016/j.jep.2011.10.027
[Indexed for MEDLINE]

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