Format

Send to

Choose Destination
Methods Mol Biol. 2012;810:119-33. doi: 10.1007/978-1-61779-382-0_8.

Fluorescence measurement of mitochondrial membrane potential changes in cultured cells.

Author information

1
Buck Institute for Research on Aging, 8001 Redwood Blvd., Novato, CA 94945-1400, USA. dnicholls@buckinstitute.org

Abstract

The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture. In this chapter, I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.

PMID:
22057564
DOI:
10.1007/978-1-61779-382-0_8
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center