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Methods Mol Biol. 2012;808:195-219. doi: 10.1007/978-1-61779-373-8_14.

Glycoarray by DNA-directed immobilization.

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1
Institut des Biomoléules Max Mousseron, UMR 5247, CNRS Université Montpellier 1, Université Montpellier 2, Montpellier, France.

Abstract

Glycoarrays have become a powerful platform to investigate the interactions of many biological events involving carbohydrates. The carbohydrates immobilization on the surface of the substrates is a key step of glycoarray fabrication. Plenty of strategies have been applied to the immobilization process. Herein a protocol for the synthesis of oligonucleotide glycomimetic conjugates is proposed. The resulting molecules are immobilized by hybridization on a DNA microarray (DNA-directed immobilization; DDI). DDI has been proved to be a very efficient and site-selective. This protocol provides detailed procedures for the preparation of fluorescent oligonucleotide trigalactosylmimetic conjugates and for the preparation of carbohydrate microarrays by DDI on glass slides.

PMID:
22057527
DOI:
10.1007/978-1-61779-373-8_14
[Indexed for MEDLINE]
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