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Allergy. 2012 Feb;67(2):210-6. doi: 10.1111/j.1398-9995.2011.02743.x. Epub 2011 Nov 5.

Cross-reactions vs co-sensitization evaluated by in silico motifs and in vitro IgE microarray testing.

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1
University Institute of Immunology, University of Bern, Switzerland.

Abstract

BACKGROUND AND OBJECTIVE:

Using an in silico allergen clustering method, we have recently shown that allergen extracts are highly cross-reactive. Here we used serological data from a multi-array IgE test based on recombinant or highly purified natural allergens to evaluate whether co-reactions are true cross-reactions or co-sensitizations by allergens with the same motifs.

METHODS:

The serum database consisted of 3142 samples, each tested against 103 highly purified natural or recombinant allergens. Cross-reactivity was predicted by an iterative motif-finding algorithm through sequence motifs identified in 2708 known allergens.

RESULTS:

Allergen proteins containing the same motifs cross-reacted as predicted. However, proteins with identical motifs revealed a hierarchy in the degree of cross-reaction: The more frequent an allergen was positive in the allergic population, the less frequently it was cross-reacting and vice versa. Co-sensitization was analyzed by splitting the dataset into patient groups that were most likely sensitized through geographical occurrence of allergens. Interestingly, most co-reactions are cross-reactions but not co-sensitizations.

CONCLUSIONS:

The observed hierarchy of cross-reactivity may play an important role for the future management of allergic diseases.

[Indexed for MEDLINE]

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