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Biochimie. 2012 Jan;94(1):263-7. doi: 10.1016/j.biochi.2011.10.008. Epub 2011 Oct 25.

Molecular identification of the sperm selection involved porcine sperm binding glycoprotein (SBG) as deleted in malignant brain tumors 1 (DMBT1).

Author information

1
Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Área Biología, Departamento de Ciencias Biológicas, Facultad de Ciencias Bioquímicas y Farmacéuticas (UNR) Suipacha 590-Rosario (S2002LRK), Santa fe, Argentina.

Abstract

Porcine sperm binding glycoprotein (SBG) is involved in sperm-oviduct interaction. Here we use mass spectrometry to identify SBG, finding peptides corresponding to deleted in malignant brain tumors 1 (DMBT1), at scavenger receptor cysteine-rich (SRCR) and CUB domains. RT-PCR allowed the cloning of unique sequences, belonging to porcine DMBT1. Western blot and immunofluorescence of oviductal tissues using anti-SBG and anti-hDMBT1 antibodies showed identical results. The biochemical characteristics of both proteins are coincident. We conclude that porcine SBG is an oviductal form of DMBT1, and thus assign this protein a novel location and function.

PMID:
22051378
DOI:
10.1016/j.biochi.2011.10.008
[Indexed for MEDLINE]

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