Send to

Choose Destination
J Biol Chem. 1990 Sep 25;265(27):16556-63.

Rapid insulin-stimulated accumulation of an mRNA encoding a proline-rich protein.

Author information

Howard Hughes Medical Institute Laboratories, Durham, North Carolina 27710.


By differential hybridization screening of a cDNA library derived from insulin-stimulated cells, we selected a clone which hybridized to an mRNA species that rapidly accumulated in response to insulin. The insert from this clone encoded a putative polypeptide of Mr 33,600, pI 11.2; because the protein was enriched in proline residues (14.4 mol %) and contained three Pro-Pro-Pro-Pro repeats, we have tentatively labeled it tris-tetraprolin (TTP). The function of this protein is not known, but it contains two regions very rich in proline (30-40 mol %); similar proline-rich regions have been shown to be involved in transcriptional activation by other proteins. The mRNA (2.0 kilobases) encoding the TTP protein was essentially undetectable in serum-deprived HIR 3.5 cells, but accumulated dramatically within 10 min of stimulation by insulin. This effect appeared to be due to insulin acting through the intrinsic protein-tyrosine kinase activity of its own receptor. Insulin induction of TTP mRNA accumulation was prevented by actinomycin D and superinduced by cycloheximide. Accumulation of TTP mRNA was also stimulated by a variety of growth factors and active phorbol esters; however, the insulin effect was virtually normal in cells depleted of protein kinase C. A single TTP gene appeared to be present in the mouse genome. This gene joins the group of genes whose members are rapidly transcribed in response to insulin and other mitogens.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center