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BMC Res Notes. 2011 Oct 31;4:470. doi: 10.1186/1756-0500-4-470.

Cyclic stretch increases splicing noise rate in cultured human fibroblasts.

Author information

1
Institute of Human Genetics, University of Ulm, Albert Einstein Allee 11, D 89070 Ulm, Germany. dieter.h.kaufmann@uni-ulm.de.

Abstract

BACKGROUND:

Mechanical forces are known to alter the expression of genes, but it has so far not been reported whether they may influence the fidelity of nucleus-based processes. One experimental approach permitting to address this question is the application of cyclic stretch to cultured human fibroblasts. As a marker for the precision of nucleus-based processes, the number of errors that occur during co-transcriptional splicing can then be measured. This so-called splicing noise is found at low frequency in pre-mRNA splicing.

FINDINGS:

The amount of splicing noise was measured by RT-qPCR of seven exon skips from the test genes AATF, MAP3K11, NF1, PCGF2, POLR2A and RABAC1. In cells treated by altered uniaxial cyclic stretching for 18 h, a uniform and significant increase of splicing noise was found for all detectable exon skips.

CONCLUSION:

Our data demonstrate that application of cyclic stretch to cultured fibroblasts correlates with a reduced transcriptional fidelity caused by increasing splicing noise.

PMID:
22040907
PMCID:
PMC3220655
DOI:
10.1186/1756-0500-4-470
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