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Nat Methods. 2011 Oct 30;9(1):64-7. doi: 10.1038/nmeth.1747.

Visualizing mechanical tension across membrane receptors with a fluorescent sensor.

Author information

1
Department of Chemistry, Emory University, Atlanta, Georgia, USA.

Abstract

We report a fluorescence-based turn-on sensor for mapping the mechanical strain exerted by specific cell-surface proteins in living cells. The sensor generates force maps with high spatial and temporal resolution using conventional fluorescence microscopy. We demonstrate the approach by mapping mechanical forces during the early stages of regulatory endocytosis of the ligand-activated epidermal growth factor receptor (EGFR).

PMID:
22037704
DOI:
10.1038/nmeth.1747
[Indexed for MEDLINE]

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