Format

Send to

Choose Destination
J Magn Reson Imaging. 2012 Mar;35(3):703-10. doi: 10.1002/jmri.22855. Epub 2011 Oct 26.

Ultrahigh-field DCE-MRI of angiogenesis in a novel angiogenesis mouse model.

Author information

1
Atherosclerosis Research Unit, Institute of Clinical Medicine and Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark. Wittenborn@oncology.dk

Abstract

PURPOSE:

To be able to screen and identify potential candidate agents for noninvasive imaging of diseases involving angiogenesis, a standardized in vivo angiogenesis model is needed. Angiogenesis is a common feature of many pathological conditions and has become an important target for diagnosis and treatment, with many noninvasive imaging agents emerging.

MATERIALS AND METHODS:

Uniform scaffolds consisting of porous and flexible polycaprolactone were implanted subcutaneously in mice and studied after 1 to 6 weeks to describe the time course of angiogenesis. The model was characterized by histology and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI).

RESULTS:

Microscopic examination revealed progressive ingrowth of new vessels from the periphery, leading to a fully vascularized scaffold within 6 weeks. Blood flow through the new vessels, assessed by DCE-MRI, revealed peripheral vascularization corresponding to 12.3% (SD 6.1%) of scaffold area at week 1 and a more uniform and complete distribution of vessels corresponding to 84.1% (SD 16.2%) of scaffold area at week 4.

CONCLUSION:

In agreement with microscopic examination, noninvasive DCE-MRI visualized progressive development of new vessels in a novel and standardized murine angiogenesis model, making this model suitable for screening angiogenesis-related drugs and contrast agents.

PMID:
22031493
DOI:
10.1002/jmri.22855
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center