Format

Send to

Choose Destination
Blood Transfus. 2011 Oct;9(4):425-9. doi: 10.2450/2011.0025-11.

Interlaboratory study to evaluate the performance of laboratories involved in West Nile virus RNA screening of blood and blood components by nucleic acid amplification testing in Italy.

Author information

1
National Centre for Immunobiologicals Research and Evaluation, ISS, Rome, Italy. giulio.pisani@iss.it

Abstract

BACKGROUND:

An Italian interlaboratory study was run in 2010 to assess the performance of Blood Transfusion Services in detecting the genome of West Nile virus (WNV) in plasma.

MATERIALS AND METHODS:

Each laboratory received a panel of samples containing four samples negative for WNV and six positive samples with a nominal viral concentration close to or below the 95% detection limit of two commercially available nucleic acid amplification tests (NAT) for WNV, the PROCLEIX® WNV kit and the Cobas® TaqScreen West Nile Virus kit.

RESULTS:

Ten laboratories took part in the study. All correctly identified the positive samples with a viral concentration above the 95% detection limit. No pre- or post-analytical errors were observed.

CONCLUSIONS:

The interlaboratory study run in 2010 allowed participants to assess the performance of the NAT methods applied in their seasonal routine screening of blood donations.

PMID:
22031284
PMCID:
PMC3200413
DOI:
10.2450/2011.0025-11
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Data Conversion Labs Icon for PubMed Central
Loading ...
Support Center