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Braz J Otorhinolaryngol. 2011 Sep-Oct;77(5):605-10.

In vitro effect of glucocorticoids on nasal polyps.

[Article in English, Portuguese]

Abstract

Glucocorticoids are considered the main treatment option for nasal polyps, but their effect is only recently being understood.

AIM:

To evaluate whether fluticasone propionate (FP) inhibits the inflammatory process induced by TNF-alpha in vitro, and to assess if NF-kappaB is associated to this inhibition.

STUDY DESIGN:

Experimental in vitro study.

MATERIALS AND METHODS:

Nasal polyp fibroblasts were cultured during 24 hours. Three different concentrations of FP (1, 10 and 100 nM, added to TNF-alpha) were compared to negative (without additive) and positive (TNF-alpha) controls. Gene expression (RTQ-PCR) and protein concentration (ELISA) of VCAM-1, ICAM-1, eotaxin and RANTES were measured, as well as the nuclear translocation of NF-kappaB.

RESULTS:

TNF-alpha significantly increased protein concentration and RNA expression of all the studied molecules, as well as the nuclear translocation of NF-kappaB, when compared to the negative control. FP decreased these parameters in a dose-dependent manner, statistically different from positive control up to 100nM.

CONCLUSIONS:

FP extensively inhibited inflammatory recruiters, at both protein and RNA levels, confirming the ability of glucocorticoids to modulate the inflammatory process in nasal polyps. This inhibition was associated to decreased NF-kappaB nuclear translocation, demonstrating that this is an important mechanism of glucocorticoids action for nasal polyps.

PMID:
22030969
[Indexed for MEDLINE]
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