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J Biomed Opt. 2011 Oct;16(10):106014. doi: 10.1117/1.3646209.

In vivo two-photon microscopy to 1.6-mm depth in mouse cortex.

Author information

1
Cornell University, School of Applied and Engineering Physics, Ithaca, New York 14853, USA. dk287@cornell.edu

Abstract

Deep tissue in vivo two-photon fluorescence imaging of cortical vasculature in a mouse brain using 1280-nm excitation is presented. A record imaging depth of 1.6 mm in mouse cortex is achieved in vivo, approximately reaching the fundamental depth limit in scattering tissue.

PMID:
22029361
DOI:
10.1117/1.3646209
[Indexed for MEDLINE]

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