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J Biomed Opt. 2011 Oct;16(10):100502. doi: 10.1117/1.3640808.

In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography.

Author information

1
Politecnico di Milano, Dipartimento di Fisica, Piazza Leonardo da Vinci 32, Milan, 20133, Italy. andreabassi@polimi.it

Abstract

We introduce flow optical projection tomography, an imaging technique capable of visualizing the vasculature of living specimens in 3-D. The method detects the movement of cells in the bloodstream and creates flow maps using a motion-analysis procedure. Then, flow maps obtained from projection taken at several angles are used to reconstruct sections of the circulatory system of the specimen. We therefore demonstrate an in vivo, 3-D optical imaging technique that, without the use of any labeling, is able to reconstruct and visualize the vascular network of transparent and weakly scattering living specimens.

PMID:
22029341
DOI:
10.1117/1.3640808
[Indexed for MEDLINE]

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