Abstract
The maturation process of [NiFe] hydrogenases includes a proteolytic cleavage of the large subunit. We constructed a mutant of Nostoc strain PCC 7120 in which hupW, encoding a putative hydrogenase-specific protease, is inactivated. Our results indicate that the protein product of hupW selectively cleaves the uptake hydrogenase in this cyanobacterium.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Catalytic Domain
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Endopeptidases / genetics
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Endopeptidases / metabolism*
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Gene Knockout Techniques
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Nostoc / enzymology*
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Nostoc / genetics
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Oxidoreductases / metabolism*
Substances
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Bacterial Proteins
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Oxidoreductases
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uptake hydrogenase
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Endopeptidases