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Appl Environ Microbiol. 2012 Jan;78(1):280-3. doi: 10.1128/AEM.05597-11. Epub 2011 Oct 21.

Expression vectors for Acinetobacter baylyi ADP1.

Author information

1
Emory University School of Medicine, Department of Biochemistry, Center for Fundamental and Applied Molecular Evolution, Atlanta, Georgia, USA.

Abstract

Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous recombination, so it can be transformed without restriction digests or ligation reactions. Expression vectors for this system, however, are not yet widely available. Here we describe the construction and characterization of inducible expression vectors that replicate as plasmids in A. baylyi or integrate into a nonessential part of its chromosome. These tools will facilitate the engineering of genes and genomes in this promising model organism.

PMID:
22020504
PMCID:
PMC3255645
DOI:
10.1128/AEM.05597-11
[Indexed for MEDLINE]
Free PMC Article

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