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Nat Methods. 2011 Oct 23;8(12):1037-40. doi: 10.1038/nmeth.1740.

NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes.

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1
Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia.

Abstract

NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.

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PMID:
22020065
DOI:
10.1038/nmeth.1740
[Indexed for MEDLINE]
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