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Neuron. 2011 Oct 20;72(2):285-99. doi: 10.1016/j.neuron.2011.08.028.

Nak regulates localization of clathrin sites in higher-order dendrites to promote local dendrite growth.

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1
Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan.

Abstract

During development, dendrites arborize in a field several hundred folds of their soma size, a process regulated by intrinsic transcription program and cell adhesion molecule (CAM)-mediated interaction. However, underlying cellular machineries that govern distal higher-order dendrite extension remain largely unknown. Here, we show that Nak, a clathrin adaptor-associated kinase, promotes higher-order dendrite growth through endocytosis. In nak mutants, both the number and length of higher-order dendrites are reduced, which are phenocopied by disruptions of clathrin-mediated endocytosis. Nak interacts genetically with components of the endocytic pathway, colocalizes with clathrin puncta, and is required for dendritic localization of clathrin puncta. More importantly, these Nak-containing clathrin structures preferentially localize to branching points and dendritic tips that are undergoing active growth. We present evidence that the Drosophila L1-CAM homolog Neuroglian is a relevant cargo of Nak-dependent internalization, suggesting that localized clathrin-mediated endocytosis of CAMs facilitates the extension of nearby higher-order dendrites.

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PMID:
22017988
DOI:
10.1016/j.neuron.2011.08.028
[Indexed for MEDLINE]
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