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Traffic. 2012 Feb;13(2):329-37. doi: 10.1111/j.1600-0854.2011.01305.x. Epub 2011 Nov 22.

The role of EGF receptor ubiquitination in regulating its intracellular traffic.

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1
UCL Institute of Ophthalmology, University College London, 11-43 Bath Street, London, EC1V 9EL, UK.

Abstract

Progression of activated EGF receptor (EGFR) through the endocytic pathway regulates EGFR signaling. Here we show that a non-ubiquitinated EGFR mutant, unable to bind the endosomal-sorting complex required for transport (ESCRT) component, Hrs, is not efficiently targeted onto intraluminal vesicles (ILVs) of multivesicular endosomes/bodies (MVBs). Moreover, ubiquitination and ESCRT engagement of activated EGFR are required for EGF-stimulated ILV formation. Non-ubiquitinated EGFRs enter clathrin-coated tubules emanating from MVBs and show enhanced recycling to the plasma membrane, compared to wild-type EGFR.

PMID:
22017370
PMCID:
PMC3261333
DOI:
10.1111/j.1600-0854.2011.01305.x
[Indexed for MEDLINE]
Free PMC Article

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